C18 Column Agilent

C18 Column Agilent

C18 Column adalah kolom HPLC fase terbalik atau reverse phase yang memiliki rantai karbon yang terikat pada partikel silika di dalam kolom. angka 18 pada C18 Column memberi tahu Anda berapa banyak rantai karbon yang terdapat pada column itu, dalam hal ini ada 18 atom karbon di setiap rantai.

Jenis fase ini adalah hidrofobik dan molekul non polar akan berinteraksi dengannya ketika mereka melewati kolom. Jadi jika Anda memiliki sampel yang memiliki dua komponen dan dimana satu komponen lebih hidrofobik daripada yang lain, komponen yang  sifat hidrofobik paling rendah akan dielusi (dilewatkan kolom) terlebih dahulu diikuti oleh molekul yang lebih hidrofobik.

Untuk membantu molekul-molekul ini terelusi atau dipisahkan campuran komponen atau senyawa dari kolom, fase gerak yang mengandung pelarut yang tinggi digunakan agar fase gerak lebih hidrofobik daripada kolom, ini akan menyebabkan molekul-molekul kurang berinteraksi dengan kolom dan tetap berada dalam fase gerak lebih lama, sampai mereka akhirnya keluar dari kolom dan kemudian melakukan perjalanan ke detektor untuk analisis.

Apa perbedaan antara C18 Column dan C8 yang digunakan dalam sistem HPLC?

Octadecylsilane (C18) memiliki 18 karbon dan octylsilane C (8) memiliki 8 atom karbon dalam struktur kolom yang diisi dengan silika. Jika komponen serupa dielusi di kedua kolom maka akan lebih cepat di C8 dan lebih lambat di C18 karena kepadatan luas permukaan kolom. C18 lebih padat dari C8 dan pengemasan lebih padat dari kolom menambah luas permukaan yang mengarahkan fase gerak untuk melakukan perjalanan per unit panjangnya. Ketika waktu retensi pendek diperlukan maka kolom C8 lebih disukai.

C18 memiliki 18 atom karbon sedangkan C8 memiliki 8 karbon dalam kemasan kolom yang terikat pada silika (Si).

Dari apa yang saya lihat secara praktis, secara umum, C18 mempertahankan lebih dari C8 sehingga untuk senyawa serupa dielusi pada dua kolom, ia akan terelusi nanti pada C18.

Ini karena kepadatan pengepakan per satuan luas permukaan kolom. Pengemasan yang lebih padat ini meningkatkan luas permukaan yang harus dilalui sebuah molekul dalam fase gerak per satuan panjang. Ini juga meningkatkan waktu interaksi antara elutes dan fase stasioner yang memberikan kemampuan pemisahan yang lebih baik untuk molekul yang lebih kompleks.

Dalam praktiknya, kolom C8 memberikan puncak yang lebih tajam daripada C18 yang menyiratkan pelat teoritis (N) yang lebih baik.

~Fred Rugenyi, B.Sc. Analytical Chemistry, Jomo Kenyatta University

Jenis Column HPLC type L berdasarkan USP List

LPACKINGBRAND NAMEMANUFACTURER
L1Octadecyl silane chemically bonded to
porous or non-porous silica or ceramic
micro-particles, 1.5 to 10 µm in
diameter, or a monolithic rod
ZORBAX ODS C18
ZORBAX ODS Classic
ZORBAX Eclipse Plus C18
ZORBAX Eclipse XDB-C18
ZORBAX Extend C18
ZORBAX Rx C18
Agilent Technologies
L2Octadecyl silane chemically bonded to
silica gel of a controlled surface
porosity that has been bonded to a solid
spherical core, 30 to 50 µm in diameter
L3Porous silica particles, 1.5 to 10 µm in
diameter, or a monolithic silica rod.
ZORBAX SIL
ZORBAX Rx-SIL
Agilent Technologies
L4Silica gel of controlled surface porosity
bonded to a solid spherical core, 30 to
50 µm in diameter
L5Alumina of controlled surface porosity
bonded to a solid spherical core, 30 to
50 µm in diameter
L6Strong cation-exchange packingsulfonated fluorocarbon polymer
coated on a solid spherical core, 30 to
50 µm in diameter
L7Octylsilane chemically bonded to
totally or superficially porous silica
particles, 1.5 to 10 µm in diameter, or a
monolithic silica rod.
Polaris C8-A
Polaris C8-Ether
ZORBAX C8
ZORBAX Eclipse Plus C8
ZORBAX Eclipse XDB-C8
ZORBAX Rx-C8
ZORBAX StableBondC8
ZORBAX SB C8
Agilent Technologies
L8An essentially monomolecular layer of
aminopropylsilane chemically bonded
to totally porous silica gel support, 1.5
to 10 µm in diameter, or a monolithic
silica rod.
ZORBAX Carbohydrate
ZORBAX NH2
Agilent Technologies
L9Irregular or spherical, totally porous
silica gel having a chemically bonded,
strongly acidic cation-exchange
coating, 3 to 10 µm in diameter.
ZORBAX 300SCX Agilent Technologies
L10Nitrile groups chemically bonded to
porous silica particles, 1.5 to 10 µm in
diameter, or a monolithic silica rod.
ZORBAX 300StableBond-CN
ZORBAX CN
ZORBAX StableBond-CN
ZORBAX Eclipse XDB-CN
Agilent Technologies
L11Phenyl groups chemically bonded to
porous silica particles, 1.5 to 10 µm in
diameter, or a monolithic silica rod.
ZORBAX Eclipse XDB-Phenyl
ZORBAX Eclipse Plus Phenyl-Hexyl
ZORBAX Phenyl
ZORBAX StableBond-Phenyl
Agilent Technologies
L12A strong anion-exchange packing made
by chemically bonding a quartenary
amine to a solid silica spherical core,
30 to 50 µm in diameter
Guard SAXAgilent Technologies
L13Trimethylsilane chemically bonded to
porous silica particles, 3 to 10 µm in
diameter
ZORBAX TMSAgilent Technologies
L14Silica gel having a chemicallly bonded
strongly basic quaternary ammonium
anion-exchange coating, 5 to 10 µm in
diameter.
ZORBAX SAXAgilent Technologies
L15Hexylsilane chemically bonded to
totally porous silica particles, 3 to 10
µm in diameter
L16Dimethylsilane chemically bonded to
porous silica particles, 5 to 10 µm in
diameter
L17Strong cation-exchange resin consisting
of sulfonated cross-linked styrenedivinylbenzene copolymer in the
hydrogen form, 6 to 12 µm in diameter
Hi-Plex H Agilent Technologies
L18Amino and cyano groups chemically
bonded to porous silica particles, 3 to
10 µ in diameter
L19Strong cation-exchange resin consisting
of sulfonated cross-linked styrenedivinylbenzene copolymer in the
calcium form, about 9 µm in diameter
L20Dihydroxypropane groups chemically
bonded to porous silica or hybrid
particles, 1.5 to 10 µm in diameter, or a
monolithic silica rod.
L21 A rigid, spherical styrenedivinylbenzene copolymer, 3 to 30 µm
in diameter
PLRP-S 100A
PLRP-S 1000A
PLRP-S 300A
PLRP-S 4000A
Agilent Technologies
L22A cation-exchange resin made of
porous polystyrene gel with sulfonic
acid groups, about 10 µm in size
Hi-Plex H
Agilent Technologies
L23An anion-exchange resin made of
porous polymethacrylate or
polyacrylate gel with quartenary
ammonium groups, 7 - 12 µm in size
L24Polyvinylalcohol chemically bonded to
porous silica particles, 5 µm in
diameter
L25Packing having the capacity to separate
compounds with a molecular weight
range from 100-5000 (as determined by
polyethylene oxide), applied to neutral,
anionic, and cationic water-soluble
polymers. A polymethacrylate resin
base, cross-linked with
polyhydroxylated ether (surface
contained some residual carboxyl
functional groups) was found suitable
L26Butyl silane chemically bonded to
totally porous silica particles, 1.5 to 10
µm in diameter
L27Porous silica particles, 30 to 50 µm in
diameter
L28A multifuncional support, which
consists of a high purity, 100 Ǻ,
spherical silica substrate that has been
bonded with anionic exchanger, amine
funcionatily in addition to a
conventional reversed phase C8
funcionality
L29Gamma alumina, reverse-phase, low
carbon percentage by weight, aluminabased polybutadiene spherical particles,
5 µm in diameter with a pore volume of
80 Ǻ units
L30Ethyl silane chemically bonded to
totally porous silica particles, 3 to 10
µm in diameter
L31A hydroxide-selective, strong anionexchange resin-quaternary amine
bonded on latex particles attached to a
core of 8.5-µm macroporous particles
having a pore size of 2000 Ǻ units and
consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene
L32A chiral ligand-exchange resin
packing-L-proline copper complex
covalently bonded to irregularly shaped
silica particles, 5 to 10 µm in diameter
L33Packing having the capacity to separate
dextrans by molecular size over a range
of 4,000 to 500,000 Da. It is spherical,
silica-based, and processed to provide
pH stability
L34Strong cation-exchange resin consisting
of sulfonated cross-linked styrenedivinylbenzene copolymer in the lead
form, 7 to 9 µm in diameter
L35A zirconium-stabilized spherical silica
packing with a hydrophilic (diol-type)
molecular monolayer bonded phase
having a pore size of 150 Ǻ.
ZORBAX GF-250
ZORBAX GF-450
Agilent Technologies
L36A 3,5-dinitrobenzoyl derivative of Lphenylglycine covalently bonded to 5-
µm aminopropyl silica
L37Packing having the capacity to separate
proteins by molecular size over a range
of 2,000 to 40,000 Da. It is a
polymethacrylate gel
L38A methacrylate-based size-exclusion
packing for water-soluble samples
L39A hydrophilic
polyhydroxymethacrylate gel of totally
porous spherical resin
L40Cellulose tris-3,5-
dimethylphenylcarbamate coated
porous silica particles, 5 µm to 20 µm
in diameter
L41Immobilized α1-acid glycoprotein on
spherical silica particles, 5 µm in
diameter
L42Octylsilane and octadecylsilane groups
chemically bonded to porous silica
particles, 5 µm in diameter
L43Pentafluorophenyl groups chemically
bonded to silica particles by a propyl
spacer, 1.5 to 10 µm in diameter
L44A multifuncional support, which
consists of a high purity, 60 Ǻ,
spherical silica substrate that has been
bonded with a cationic exchanger,
sulfonic acid funcionality in addition to
a convention reversed phase C8
funcionality
L45Beta cyclodextrin, R,S-hydroxypropyl
ether derivative, bonded to porous
silica particles, 5 to 10 µm in diameter
L46Polystyrene/divinylbenzene substrate
agglomerated with quaternary amine
funcionalized latex beads, about 9 µm
to 11 µm in diameter
L47High capacity anion-exchange
microporous substrate, fully
functionalized with a trimethylamine
group, 8 µm in diameter
L48Sulfonated, cross-linked polystyrene
with an outer layer of submicron,
porous, anion-exchange microbeads, 5
to 15 µm in diameter
L49A reversed-phase packing made by
coating a thin layer of polybutadiene on
to spherical porous zirconia particles, 3
to 10 µm in diameter
L50Multifunction resin with reverse-phase
retention and strong anion-exchange
functionalities. The resin consists of
ethylvinylbenzene, 55% cross-linked
with divinylbenzene copolymer, 3 to 15
µm in diameter, and a surface are of not
less than 350 m2
per g. Substrate is
coated with quartenary ammonium
functionalized latex particles consisting
of styrene cross-linked with
divinylbenzene
L51Amylose tris-3,5-
dimethylphenylcarbamate-coated,
porous, spherical, silica particles, 5 to
10 µm in diameter
L52A strong cation exchange resin made of
porous silica with sulfopropyl groups, 5
to 10 µm in diameter
L53Weak cation-exchange resin consisting
of ethylvinylbenzene, 55% cross-linked
with divinylbenzene copolymer, 3 to 15
µm diameter. Substrate is surface
grafted with carboxylic acid and/or
phosphoric acid functionalized
monomers. Capacity not less than 500
µEq/column
L54A size exclusion medium made of
covalent bonding of dextran to highly
cross-linked porous agarose beads,
about 13 µ in diameter.
L55A strong cation exchange resin made of
porous silica coated with
polybutadiene-maleic acid copolymer,
about 5 µm in diameter
L56Propyl silane chemically bonded to
totally porous silica particles, 3 to 10
µm in diameter
ZORBAX StableBond C3 Agilent Technologies
L57A chiral-recognition protein,
ovomucoid, chemically bonded to silica
particles, about 5 µm in diameter, with
a pore size of 120 Ǻ.
Ultron ES-OVM
Ultron ES-OVM
Agilent Technologies
L58Strong cation-exchange resin consisting
of sulfonated cross-linked styrenedivinylbenzene copolymer in the
sodium form, about 6 to 30 µm
diameter
L59Packing for the size-exclusion
separations of proteins (separation by
molecular weight) over the range of 5
to 7000 kDa. The packing is spherical
1.5 - 10 µm, silica or hybrid packing
with a hydrophilic coating.
L60Spherical, porous silica gel, 10 µm or
less in diameter, the surface of which
has been covalently modified with
alkyl amide groups and endcapped
ZORBAX Bonus-RP Agilent Technologies
L61A hydroxide-selective, strong anionexchange resin consisting of a highly
cross-linked core of 13 µm
microporous particles having a pore
size less than 10 Ǻ units and consisting
of ethylvinylbenzene cross-linked with
55% divinylbenzene with a latex
coating composed of 85 nm diameter
microbeads bonded with alkanol
quarternary ammonium ions (6%).
L62C30 silane bonded phase on a fully
porous spherical silica, 3 to 15 µm in
diameter.
L63Glycopeptide teicoplanin linked
through multiple covalent bonds to a
100 Ǻ units spherical silica
L64Strongly basic anion exchange resin
consisting of 8% crosslinked styrene
divinylbenzene copolymer with a
quartenary ammonium group in the
chloride form, 45 to 180 µm in
diameter
L65Strongly acidic cation exchange resin
consisting of 8% sulfonated crosslinked
styrene divinylbenzene copolymer with
a sulfonic acid group in the hydrogen
form, 63 to 250 µm in diameter
L66A crown ether coated on a 5 µm
particle size silica gel substrate. The
active site is (S) -18-crown-6-ether
L67Porous vinyl alcohol copolymer with a
C18 alkyl group attached to the
hydroxyl group of the polymer, 2 to 10
µm in diameter.
L68Spherical, porous silica, 10 µm or less
in diameter, the surface of which has
been covalently modified with alkyl
amide groups and not endcapped
L69Ethylvinylbenzene/divinylbenzene
substrate agglomerated with quaternary
amine functionalized 130 nm latex
beads, about 6.5 µm in diameter.
L70Cellulose tris(phenyl carbamate) coated
on 5 µm silica
L71A rigid, spherical polymetacrylate, 4 to
6 µm in diameter
L72(S)-phenylglycine and 3,5-
dinitroanaline urea linkage covalently
bonded to silica
L73A rigid, spherical polydivinylbenzene
particle, 5 to 10 µ in diameter
L74A strong anion-exchange resin
consisting of a highly cross-linked core
of 7-µm macroporous particles having
a 100 Angstroms average pore size and
consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene and an
anion-exchange layer grafted to the
surface, which is functionalized with
alkyl quartenary ammonium ions.
L75A chiral-recognition protein, bovine
serum albumin (BSA), chemically
bonded to silica particles, about 7 µm
in diameter, with a pore size of 300
Angstroms
L76Silica based weak cation-exchange
material, 5 µm in diameter. Substrate is
surface polymerized polybutadienemaleic acid to provide carboxylic acid
functionalities. Capacity not less than
29 µEq/column
L77Weak cation-exchange resin consisting
of ethylvinylbenzene, 55% cross-linked
with divinylbenzene copolymer, 6 to 9
µm diameter. Substrate is surface
grafted with carboxylic acid
functionalized groups. Capacity not
less than 500 µEq/column (4 mm x 25
cm)
L78A silane ligand that consists of both
reversed-phase (an alkyl chain longer
than C8) and anion-exchange (primary,
secondary, tertiary, or quartenary
amino groups) functional groups
chemically bonded to porous or nonporous or ceramic micro-particles, 1.0
to 50 µm in diameter or a monolithic
rod
L79A chiral-recognition protein, human
serum albumin (HSA), chemically
bondend to silica particles, about 5 µm
in diameter.
L80Cellulose tris(4-methylbenzoate)-
coated, porous, spherical, silica
particles, 5 µ in diameter
L81A hydroxide-selective, strong anionexchange resin consisting of a highly
cross-linked core of 9 µm porous
particles having a pore size of 2000
Angstroms units and consisting of
ethylvinylbenzene cross-linked with
55% divinylbenzene with a latex
coating composed of 70 nm diameter
microbeads (6% crosslinked) bonded
with alkanol quartenary ammonium
ions
L82Polyamine chemically bonded to crosslinked polyvinyl alcohol polymer, 4 - 5
µm in diameter
L83A hydroxide-selective, strong anionexchange resin-quartenary amine
bonded on latex particles attached to a
core of 10.5 µm microporous particles
having a pore size of 10 Angstroms and
consisting of ethylvinylbenzene crosslinked with 55% divinylbenzene.
L84Weak cation-exchange resin consisting
of ethylvinylbenzene, 55% cross-linked
with divinylbenzene copolymer, 5 µm
diameter. Substrate is surface grafted
with carboxylic acid functionalized
groups. Capacity not less than 8400
µEq.column (5 mm x 25 cm).
L85A silane ligand that consists of both
reversed-phase (an alkyl chain longer
than C8) and weak cation-exchange
(carboxyl groups) functional groups
chemically bonded to porous or nonporous particles, 1.0 to 50 µm in
diameter
L86A 5 µm fused core particle with a
highly polar ligand possessing 5
hydroxyl groups tethered to the silica
gel outer layer

InfinityLab Poroshell 120

InfinityLab Poroshell 120 kolom memberikan efisiensi dan keandalan yang luar biasa, secara signifikan meningkatkan kinerja dari semua instrumen, apakah Anda ingin meningkatkan kinerja HPLC, kinerja UHPLC pada tekanan rendah atau kinerja UHPLC tertinggi.

Tiga diameter partikel— 1,9 μm, 2,7 μm, dan 4 μm — memungkinkan Anda untuk memilih ukuran yang paling sesuai dengan kebutuhan pemisahan Anda dan paling kompatibel dengan sistem LC Anda, dan 12 kimia berbeda menyediakan berbagai selektivitas yang membuat pengembangan metode Anda cepat dan mudah.

ZORBAX Extend-C18

Extend-C18 kolom menggabungkan bidentate silane yang sudah dipatenkan, dikombinasikan dengan proses double-endcapping yang melindungi rusaknya silika dari pada pH tinggi – hingga pH 11,5. Kolom ini cocok dan paling baik diterapkan untuk pemisahan senyawa yang

  1. Basa dan memiliki sedikit atau tidak ada retensi pada pH rendah atau menengah,
  2. Lebih stabil atau lebih larut pada pH tinggi, atau
  3. Basa dan menunjukkan bentuk puncak yang buruk pada pH rendah atau sedang.

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